Molecular detection of Mycoplasma gallisepticum in different poultry breeds of Abbottabad and Rawalpindi, Pakistan

Abstract The poultry sector in Pakistan is contributing mainly bridging gap between demand and supply for protein. Mycoplasma gallisepticum an emerging bacterium causing serious problems industry of Pakistan. A cross-sectional study was conducted to evaluate the M. load populated regions Total 600 serum swab samples were collected, 200 from each broiler, layers breeders Rawalpindi Abbottabad districts. Serum analyzed through ELISA seroprevalence. Swabs cultured on Frey’s medium followed by PCR partial mgc2 gene sequencing. Results seroprevalence showed that (75%, n=150) are more positive as compared (70%, n=140) broilers (50%, n=100). Typical colonies observed breeder (26.5%), layer (21%) (9%). total 37.1% (n=42) identified out 113 based samples. six isolates current 98-99 percent similarity with previously reported basis found highly prevalent different breads. this would add into basic data provide a direction livestock strengthen control strategy mycoplasmosis farms.